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The science and practice of human immunology

The science and practice of human immunology
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GENERAL IMMUNOPHENOTYPING (FLOW CYTOMETRY)

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Lymphocyte Subset Enumeration T Cell Phenotyping (Naïve T Cells, Memory T Cells, Extended Phenotyping)
TCR α/β - TCR γ/δ TCR V Beta Repertoire
Regulatory T Cell Analysis and/or FOXP3 Expression CD4+ Recent Thymic Emigrants
Lymphocyte/T cell Activation NKT cell Subsets (including invariant NKT cells)
NK Cell Phenotyping B Cell Enumeration and/or Extended Phenotyping

 

Lymphocyte Subset Enumeration

ARUP
Lymphocyte Subset Panel 1: CD4 Absolute Count Only. Reported; abs CD4. By Quantitative Flow Cytometry. Test Code: 0095854

Lymphocyte Subset Panel 2: CD4 Percent and Absolute. Reported; % CD4, and Abs CD4. By Quantitative Flow Cytometry. Test Code: 0095885

Lymphocyte Subset Panel 3: T-Cell Subsets (CD4 and CD8), Absolute Counts Only. By Quantitative Flow Cytometry. Reported; Abs CD3, CD4, CD8, and abs CD4:CD8 ratio. Test Code: 0095853

Lymphocyte Subset Panel 4: T-Cell Subsets Percent and Absolute. Reported; %CD3, abs CD3, %CD4, abs CD4, %CD8, abs CD8, CD4:CD8 Ratio. By Quantitative Flow Cytometry. Test Code: 0095950

Lymphocyte Subset Panel 5: Total Lymphocyte Enumeration. Reported; % CD3, abs CD3, % CD4, abs CD4, % CD8, abs CD8, CD4: CD8 ratio, % CD19, abs CD19, % NK-cells, abs NK-cells. By Quantitative Flow Cytometry. Test Code: 0095892

Isolated Panel for T cells (Monitoring post ATG):  Lymphocyte Transplantation CD3. Reported: WBC (K/µL), % Lymph, Absolute Lymph Count, %CD3, abs CD3. By Quantitative Flow Cytometry. Test Code: 0095949

Natural Killer Cells Enumeration: The results reported are the percentage of lymphocytes that are Natural killer (NK) cells and the absolute count of NK-cells per µL of blood. Only those cells that express CD45 and either CD16 or CD56 (or both) but not CD3 or CD19 are reported. By Quantitative Flow Cytometry. Test Code: 0092404


BCH-CFCL
Analysis of lymphocytes populations, which reports: The percentages and absolute numbers of lymphocytes that are CD3+ (Total T cells), CD3+CD4+ (T helper cells), CD3+CD8+ (T cytotoxic cells), CD4:CD8 ratio, CD3-CD19+ (B cells), CD-CD16/56+ (NK cells). Test Code: Lymphocyte Subsets or T/B/NK Panel


CALGARY-CLS
Immunodeficiency screen panel includes percentages and absolute numbers of T cells, B cells, NK cells, TNK (CD3+16/56+), CD3+4+, CD3+8+, DNT (CD3+4-8-), CD4/CD8 ratio, Naïve B cells (CD19+IgD+CD27-), Memory non-class switched B cells (CD19+IgD+CD27+), Memory class switched B cells (CD19+IgD-CD27+), Naïve Helper T cells (CD3+4+CD45RA+CD27+), Terminally Differentiated Helper T cell (CD3+4+45RA+27-), Central Memory Helper T cell (CD3+4+45RA-27+), Effector Memory Helper T cell (CD3+4+45RA-27-), Naïve Cytotoxic T cells (CD3+8+CD45RA+CD27+), Terminally Differentiated Cytotoxic T cell cell (CD3+8+45RA+27-), Central Memory Cytotoxic T cell (CD3+8+45RA-27+), Effector Memory Cytotoxic T cell (CD3+8+45RA-27-). CD45RA/CD45RO also assessed. Test Code: IDEF


CCHMC-DIL
The percentages and absolute numbers of lymphocytes that are T cells (CD3+), T-helper (CD3+, CD4+), T-cytotoxic (CD3+, CD8+), natural killer (CD16+CD56+, CD3-), and B lymphocytes (CD19+) are reported.  Test Code: Lymphocyte Subsets


CHLA-DIFL
T cell Standard panel: The percentages and absolute numbers of lymphocytes that are CD3+ (Total T cells), CD3+CD4+ (T helper cells), CD3+CD8+ (T cytotoxic cells), CD4:CD8 ratio, and CD3+ HLA-DR+ (Activated T cells) are reported. Test Code: T-Cell Standard

T cell Extended panel: The percentages and absolute numbers of lymphocytes that are CD3+ (Total T cells), CD3+CD4+ (T helper cells), CD3+CD8+ (T cytotoxic cells), CD4:CD8 ratio, CD3-CD19+ (B cells), CD-CD16/56+ (Natural Killer (NK) cells), and CD3+ HLA-DR+ (Activated T cells) are reported. Test Code: T-Cell Extended


MAYO-ML
The percentages and absolute numbers of lymphocytes that are T cells (CD3+), T-helper (CD3+, CD4+), T-cytotoxic (CD3+, CD8+), natural killer (CD16+CD56+, CD3-), and B lymphocytes (CD19+) are reported.  Test Code: TBBS; #9336


MCW-CIRL
CD3, CD4, CD8, CD14, CD45. Test Code: AT4
CD3, CD4, CD8, CD16, CD56, CD19, CD14, CD15, CD11b, CD45. Test Code: PID1


MONTREAL-SJH
Lymphocyte subset phenotyping by quantitative flow cytometry:  T lymphocytes: CD3+, CD3+CD4+, CD3+CD8. Test Code: CD3, CD4, CD8
Lymphocyte subset phenotyping by quantitative flow cytometry:  NK cells: CD3-CD56+. Test Code: CD3 (-) CD56 (+)
Lymphocyte subset phenotyping by quantitative flow cytometry:  B lymphocyte subsets CD19+, CD19+CD27+, CD19+CD20+. Test Code: CD19, CD27/CD19, CD20


NJH-ADL
Lymphocyte Phenotyping Panel: CD3+, CD3+/CD4+, CD3+/CD8+, CD16/56 and CD19+. CD4/CD8 ratio reported.  The percentages and absolute numbers are reported. Test Code: TBCDC

CD3/4/8: The percentages and absolute numbers are reported. Test Code: CD3SB
CD3/4/8/19: The percentages and absolute numbers are reported. Test Code: TBSBS
CD4/8: The percentages and absolute numbers are reported. Test Code: CD48
Individual markers - CD4:  The percentage and absolute number are reported. Test Code CD4M
Individual markers - CD16/56:  The percentage and absolute number are reported. Test Code NKMK


NSUH

Lymphocyte subset uses CD3, CD4, CD8, CD19, CD16, and CD56.  Report includes percentages and absolute counts. CD4 to CD8 ratio is also reported. Test Code: TnB


UCLA-UIC
Both the T-cell memory and the B-cell panels include a basic panel for lymphocyte enumeration, which reports the percentages and absolute numbers of T cells (CD3+), T-helper (CD3+, CD4+), T-cytotoxic (CD3+, CD8+), CD4:CD8 ratio, natural killer (CD16+CD56+, CD3-), and B lymphocytes (CD19+), followed by an advanced panel to identify (i) T-memory: naïve (CD45RA+/CD45RO-) and Memory (CD45RA-/CD45RO+) CD4+ and CD8+ T cells; (ii) B-cell: Memory (CD27+), IgM Memory (CD27+IgM+IgD-), Unswitched Memory (CD27+IgM+IgD+), Switched Memory (CD19+CD27+IgD-IgM-) and Immature (CD21low) CD19+ B cells. Test Codes: T-MEM or B-CELL

 

T Cell Phenotyping (Naïve T Cells, Memory T Cells, Extended Phenotyping)

ARUP
Lymphocyte Subset Panel 6: Total Lymphocyte Enumeration with CD45RA and CD45RO. Reported; %CD3, abs CD3, % CD4, abs CD4, % CD45RA, abs CD45RA, % CD45RO, abs CD45RO, % CD8, abs CD8, CD4:CD8 Ratio, % CD19, abs CD19, % NK-cells, abs NK-cells. By Quantitative Flow Cytometry. Test Code: 0095862

Lymphocyte Subset Panel 7: Congenital Immunodeficiencies. Reported; %CD2, abs CD2,  % CD3, Abs CD3, %HLA-DR, abs HLA-DR, % CD4, abs CD4, % CD45RA, Abs CD45RA,  % CD8, abs CD8, CD4: CD8 ratio, % CD19, abs CD19, % NK-cells, abs NK-cells. By Quantitative Flow Cytometry. Test Code: 0095899


BCH-CFCL
Immunophenotyping of Naïve and Memory T cells, which reports: TEMRA CD4+CD45RA+CCR7-T cells, Naïve CD4+CD45RA+CCR7+T cells, Effector memory CD4+CD45RA-CCR7-T cells, Central memory CD4+CD45RA-CCR7+T cells, TEMRA CD8+CD45RA+CCR7-T cells, Naïve CD8+CD45RA+CCR7+T cells, Effector memory CD8+CD45RA-CCR7-T cells, Central memory CD8+CD45RA-CCR7+T cells. Test Code: T Cell Memory Panel


CALGARY-CLS
Immunodeficiency screen panel includes percentages and absolute numbers of T cells, B cells, NK cells, TNK (CD3+16/56+), CD3+4+, CD3+8+, DNT (CD3+4-8-), CD4/CD8 ratio, Naïve B cells (CD19+IgD+CD27-), Memory non-class switched B cells (CD19+IgD+CD27+), Memory class switched B cells (CD19+IgD-CD27+), Naïve Helper T cells (CD3+4+CD45RA+CD27+), Terminally Differentiated Helper T cell (CD3+4+45RA+27-), Central Memory Helper T cell (CD3+4+45RA-27+), Effector Memory Helper T cell (CD3+4+45RA-27-), Naïve Cytotoxic T cells (CD3+8+CD45RA+CD27+), Terminally Differentiated Cytotoxic T cell (CD3+8+45RA+27-), Central Memory Cytotoxic T cell (CD3+8+45RA-27+), Effector Memory Cytotoxic T cell (CD3+8+45RA-27-). CD45RA/CD45RO also assessed. Test Code: IDEF


CCHMC-DIL
Panel uses CD3, CD4, CD8, CD45RA, CD45RO, CD27, CCR7, and CD31 to identify naïve (CD45RA+, CD27+), Central Memory (CD45RO+, CCR7+), and Effector Memory (CD45RO+, CCR7-) CD4+ and CD8+ T cells, and also evaluates the expression of CD31 as a marker of recent thymic emigrants. Test Code: CD45RA/RO


CHLA-DIFL
Naïve T Cell Subsets. The percentages and absolute numbers of lymphocytes that are newly formed CD4+ cells with following phenotypes of CD4+CD45+RA+ cells: CD3+4+/RA+RO+, CD3+4+/RA+/CD31+ and CD3+4+/RA+62L+ are reported. Test Code: T cell Subsets (Memory and Naïve)


MAYO-ML
Quantitates naïve (global and CD62L+), memory (global, central, and effector memory), and activated (CD4+25+ and MHC class II-positive) CD4+ and CD8+ T cells using antibodies directed against CD3, CD4, CD8, CD45RO, CD45RA, HLADR, CD27, CD62L, CD25, and CD28. Test Code: TCP; #89319


MCW-CIRL
Primary Immunodeficiency 2 panel includes CD3, CD4, CD8, TCRαβ, CD69, CD45RA, CD45RO, CD16, CD56; CD19, CD20, CD27, CD5, CD14, CD15, CD11b, CD18, CD45, HLA-DR, HLA-ABC. Test Code: PID2

CVID Panel: CD3, CD4, CD8, CD16, CD56, CD19, CD21, CD27, IgD, HLA-DR, CD45RA, CD45RO, CD69, CD14, CD15, CD11b, CD45. Test Code: CVID  


MONTREAL-SJH
Naïve/memory T lymphocytes: CD4+CD45RO+, CD4+CD45RA+, CD4+CD45RA+CD31+/ CD8+CD45RA+CCR7+ (naïve), CD8+CD45RA-CCR7+ (CM), CD8+CD45RA-CCR7- (TEM), CD8+CD45RA+CCR7- (TEMRA). Test Code: CD45RA/CD4, CD45RA-31/CD4, CD45RO/CD4, CD45RA/CD8, CD45RA-31/CD8, CD45RO/CD8


NSUH
Naïve/memory T-Lymphocyte subset uses CD3, CD4, CD8, CD45RA, and CD45RO.  Percentages are reported. Test Code: CD45RARO


NJH-ADL
National Jewish Health runs a memory and naïve T cell panel that includes CD3/4/CD45RA/CD45RO. Test Code: C4RARO


UCLA-UIC
The T-cell memory panel includes a basic panel for lymphocyte enumeration, which reports the percentages and absolute numbers of T cells (CD3+), T-helper (CD3+, CD4+), T-cytotoxic (CD3+, CD8+), CD4:CD8 ratio, natural killer (CD16+CD56+, CD3-), and B lymphocytes (CD19+), followed by an advanced panel to identify naïve (CD45RA+/CD45RO-) and Memory (CD45RA-/CD45RO+) CD4+ and CD8+ T cells. Test Code: T-MEM

 

TCR α/β - TCR γ/δ

BCH-CFCL
Immunophenotyping of γδ and CD4-CD8- αβ T cells, which reports: CD3+CD4-CD8-TCR alpha/beta T cells, CD3+TCR gamma/delta T cells. See also ALPS panel. Test Code: ALPS Panel


CALGARY-CLS
Reports the % and absolute count of TCRα/β and TCRγ/δ positive T cells: Test Code: TCRABGD


CCHMC-DIL
Quantifies the percentages of T cells expression α/β TCR and γ/δ TCR. Test Code: TCR α/β TCR γ/δ


CHLA-DIFL
Quantifies the percentages of T cells expression α/β TCR and γ/δ TCR. Test Code: TCR α/β TCR γ/δ


MONTREAL-SJH
Percentages of TCR α/β /CD3 and TCR γ/δ /CD3. Test Code: TCR α/β TCR γ/δ /CD3


NJH-ADL
Quantifies the percentages of TCRα/β and γ/δ T cells. Test Code: TCABGD


NSUH
T-lymphocyte TCR subset uses CD3, CD4, CD8, TCRα/β, and TCRγ/δ.  Percentages are reported. Test Code: TCR

 

TCR V Beta Repertoire

ARUP
T-Cell Clonality by Flow Cytometry Analysis of TCR V-beta. Quantitates 24 alpha-beta T cell antigen receptors. Test Code: 0093199


CALGARY-CLS
Provides % and graphic representation of 24 vbeta repertoire antigens on CD3, CD3+4+ and CD3+8+ subsets. Test Code: TCR FLOW


CCHMC-DIL
Quantitates 24 antigens of the TCR V β repertoire of T lymphocytes. Test Code: TCR V Beta Repertoire


CHLA-DIFL
TCR V Beta Repertoire. Quantitates 24 V Beta TCR family receptors in total CD3, CD4, and CD8 T-cell subsets. Test Code:  


MAYO-ML
See Molecular Tests: TCR Vb Spectratyping


MONTREAL-SJH
Percentages of TCR Vb repertoire  (flow cytometry) in CD4+ and CD8+ T cells. Test Code: V Beta 2/CD4, V Beta 2/CD8, 6 V Beta /CD4, 6 V Beta /CD4


QUEST
See Molecular Tests: TCR Vb Spectratyping

 

Regulatory T Cell Analysis and/or FOXP3 Expression

ARUP
Regulatory T-Cell Panel. Reported: Percent and absolute numbers of CD4+ CD25+ CD127Low (Tregs), and CD45RA and CD45RO also included in the panel. By Quantitative Flow Cytometry.  Test Code: 2010172


BCH-CFCL
Immunophenotyping of Regulatory T cells, which reports: CD4+CD25+CD127+ T cells, CD4+CD25highCD127low T cells. Test Code: Treg Panel


CALGARY-CLS
Reports the % and absolute count of Tregs determined by CD3+ CD4+ lowCD127+ and CD25+. Test Code: TREG


CCHMC-DIL
Quantifies the percent of T regs (CD25+ CD127 lo) as a percentage of the CD4+ T cells, and quantifies the percentage of T regs, which express FOXP3. Test Code: FoxP3


CHLA-DIFL
Regulatory T-Cell (T Reg). Percent of CD4+ CD25+ CD127dim is reported. Test Code:  


NJH-ADL
National Jewish Health using a 4-color panel, CD3/4/CD25/ FoxP3, gating on CD25highFoxP3+ cells. Reports percentages. Test Code: FOXP3


MCW-CIRL
Treg panel includes: CD4, CD25, CD127, FoxP3. Test Code: TREG


MAYO-ML
Identifies percentages and absolute numbers of CD4+CD25+CD127loCD45RO+ (Nat Tregs) and CD4+CD25+CD127loCD45RA+ Naive Tregs. Test Code: TREGS; #89318


MONTREAL-SJH
Percentages of regulatory T cells: CD4+ CD25high CD127- Foxp3+ by flow cytometry. Test Code: FOXp3


NSUH
Regulatory T-Lymphocytes uses CD4, CD25, CD127, and FoxP3.  Percentages are reported. Test Code: FoxP3

 

CD4+ Recent Thymic Emigrants

ARUP
CD4+ T-Cell Recent Thymic Emigrants (RTEs). Reports percentage and absolute numbers of CD4+CD31+CD45RA+ (RTEs). By Quantitative Flow Cytometry. Test Code: 2010179


BCH-CFCL
Immunophenotyping of Recent Thymic Emigrants, which reports: CD4+CD31+CD45RA+Recent Thymic Emigrants. Test Code: RTE Panel


CALGARY-CLS
Reports the % and absolute count of CD3+CD4+CD45RA+CD31+ recent thymic emigrants. Test Code: RTE


CCHMC-DIL
CD45RA/RO panel includes evaluation of CD31 expression as a marker of recent thymic emigrants. Test Code: CD45RA/RO


CHLA-DIFL
The percentages and absolute numbers of lymphocytes that are newly formed CD4+ cells with following phenotypes of CD4+CD45+RA+ cells: CD3+4+/RA+RO+, CD3+4+/RA+/CD31+ and CD3+4+/RA+62L+ are reported. Test Code: T Cell Subsets (Memory and Naïve)


MAYO-ML
Reports the percentage of CD4+ 45RA+ CD31+ T cells. Test Code: CD4RT; #89504


MONTREAL-SJH
Naïve/memory T lymphocyte subsets: CD4+CD45RO+, CD4+CD45RA+, CD4+CD45RA+ CD31+, and CD8+ CD45RA+ CCR7+ (naïve), CD8+CD45RA-CCR7+ (CM), CD8+CD45RA-CCR7- (TEM), CD8+CD45RA+CCR7- (TEMRA). Test Code: CD45RA/CD4, CD45RA-31/CD4, CD45RO/CD4, CD45RA/CD8, CD45RA-31/CD8, CD45RO/CD8


NJH-ADL
Identifies RTEs using a 4-color panel: CD3/CD4/CD45RA/CD31. Reports percentages. Test Code: CD4RTE

 

Lymphocyte/T cell Activation

CALGARY-CLS
Panel 1:  CD3+4+ T cells are assessed for surface expression of CD154, CD69, HLA-DR, CD143, CD25, CD71, and CD96 pre and post PMA/Ionomycin stimulation.  Panel 2: CD8+ T cells are assessed for expression of CD45RO and HLA-DR primarily for use in investigation of Omenn’s Syndrome or HLH.  Antigen density of CD5 on CD3+8+ cells is also reported for HLH investigations. Test Code: LAM


CCHMC-DIL
Measures CD4+ T cell surface expression of CD69, CD25, CD154, CD134, CD95, CD71, and HLA-DR. Test Code: Lymphocyte Activation Markers


CHLA-DIFL
T Cell Activation Panel. The following populations are reported in percentages: CD25+CD3+/CD3, DR+CD3+/CD3, CD69+CD3+/CD3, CD134+CD3+/CD3, CD25+CD4+/CD3, DR+CD4+/CD3, CD69+CD4+/CD3, CD134+CD4+/CD3. Test Code: T Cell Subsets Activated T


MAYO-ML
The T cell phenotyping assay includes evaluation of activated (CD4+25+ and MHC class II-positive) CD4+ and CD8+ T cells. Test Code: TCP; #89319


MCW-CIRL
T cell activation panel: Includes CD4, CD8, CD69, and CD25 at 4 hours and 4 days after activation to measure early and late activation events: Test Code: TCACT


MONTREAL-SJH
Sainte Justine Hospital: Percentages of activated T cells for HLA class II DR expression on CD3+, CD3+CD4+, and CD3+CD8+ by flow cytometry. Test Code: HLA class II/CD3, HLA class II/CD4, HLA class II/CD8

 

NKT cell Subsets (including invariant NKT cells)

CALGARY-CLS
The percentage and absolute count of CD3+ TCRvalpha24+ TCRvbeta11+ iNKT cells is reported. Test Code: INKT


CCHMC-DIL
Quantification of TCRValpa24 and TCR Vbeta 11 expression on invariant NKT cells. Test Code: iNKT


MONTREAL-SJH
Quantification of MAIT (Va 24 Vb 11) and iNKT (Va 24 Vb 11) cells. Test Code: NKT

 

NK Cell Phenotyping

ARUP
Natural Killer Cells Enumeration. The results reported are the percentage of lymphocytes that are Natural killer (NK) cells and the absolute count of NK-cells per µL of blood. Only those cells that express CD45 and either CD16 or CD56 (or both) but not CD3 or CD19 are reported. By Quantitative Flow Cytometry. Test Code: 0092404

Natural Killer Cell and Natural Killer T-Cell Panel. The panel reports frequencies and absolute cell counts of NK and NKT-cells, also including T and non-T cells: CD3-CD16-/+CD56bright/dim (total NK-cells), CD3-CD16+CD56dim (cytotoxic NK-cells), CD3-CD16-CD56bright (cytokine secreting NK-cells), CD3-CD57+ (CD57+NK-cells), CD3+CD56+ (CD56+ NKT-cells), CD3+CD57+ (CD57+ NKT-cells), CD45+CD3+ (T-cells) and CD45+CD3- (Non T-cells). Test Code: 2013805


BCH-CFCL
Immunophenotyping of Natural Killer cells which reports: percentages of CD56 high NK cells, CD56+CD16+ NK cells, and CD56-CD16 + NK cells. Test Code: NK Cell Phenotype


MAYO-ML
Includes quantitation of cytotoxic and cytokine-producing NK cells based on expression and intensities of CD16 and CD56, reports CD3+ CD56+.T cells, and includes CD69, CD27, NKp46, NKG2D, CD95 CD107a/b, Perforin, Granzyme A, Granzyme B, and IFNg. Test Codes: QNKS; #60616 (for only the major NK/NKT cell subset quant without intracellular and surface markers; NKSP (for complete panel) #60615


MCW-CIRL
NK panel includes: CD3, CD4, CD8, CD56, Perforin, Granzyme A, Granzyme B, CD19, CD14, CD15, CD11b, CD45. Test Code: PERGRA

 

B Cell Enumeration and/or Extended Phenotyping

ARUP
B-Cell CD20 Expression. Reported; %CD19, %CD20, and interpretation. Used primarily to monitor response to rituximab therapy. Test Code: 0092099

B-Cell Memory and Naïve Panel. Subsets measured: B-cells (CD19+), total memory B-cells (CD19+ CD27+), class switched memory B-cells (CD19+ CD27+ IgD-IgM-), non-switched/marginal zone memory B-cells (CD19+ CD27+ IgD+ IgM+), IgM only memory B-cells (CD19+ CD27+ IgD-IgM+), and naïve B-cells (CD19+ CD27-IgD+). Test Code: 2008901


BCH-CFCL

Immunophenotyping of Naïve and Memory B Cells, which reports: Switched memory IgD-CD27+B cells, Unswitched memory IgD+CD27+B cells, Naïve IgD+CD27-B cells. Test Code: B Cell Memory Panel


CALGARY-CLS
Immunodeficiency screen panel includes percentages and absolute numbers of T cells, B cells, NK cells, TNK (CD3+16/56+), CD3+4+, CD3+8+, DNT (CD3+4-8-), CD4/CD8 ratio, Naïve B cells (CD19+IgD+CD27-), Memory non-class switched B cells (CD19+IgD+CD27+), Memory class switched B cells (CD19+IgD-CD27+), Naïve Helper T cells (CD3+4+CD45RA+CD27+), Terminally Differentiated Helper T cell (CD3+4+45RA+27-), Central Memory Helper T cell (CD3+4+45RA-27+), Effector Memory Helper T cell (CD3+4+45RA-27-), Naïve Cytotoxic T cells (CD3+8+CD45RA+CD27+), Terminally Differentiated CytotoxicT cell (CD3+8+45RA+27-), Central Memory Cytotoxic T cell (CD3+8+45RA-27+), Effector Memory Cytotoxic T cell (CD3+8+45RA-27-). CD45RA/CD45RO also assessed. Test Code: IDEF


CCHMC-DIL
Panel uses CD45, CD19, CD20, CD10, CD5, CD21, CD27, CD38, CD138, IgD, and IgM to identify B cell populations. Also includes B220. Test Code: B Cell Panel


CHLA-DIFL
B Cell Subsets. The following populations are reported in percentages: CD19, IgD+CD27-CD19+/CD19, IgD+CD27+CD19+/CD19, IgD-CD27+CD19+/CD19. Test Code: B cell Extended


MAYO-ML
Percent and absolute count reported for CD19 and CD20+ B cells (for monitoring patients on rituximab). Test Code: CD20B; #89584

Assay uses CD45, CD19, CD20, CD27, IgD, IgM, CD38, and CD21 to define B cell populations.  Test Code: IABCS; #88800 (includes the T, B and NK cell quantitation, and provides % and absolute count data).  For % only data: Test Code: RBCS; #36440


MCW-CIRL
Panel includes:  CD3, CD4, CD8, CD16, CD56, CD19, CD21, CD27, IgD, HLA-DR, CD45RA, CD45RO, CD69, CD14, CD15, CD11b, CD45. Test Code: CVID  


MONTREAL-SJH
B lymphocyte subsets CD19+, CD19+CD27+, CD19+CD20+. Test Code: CD19, CD27/CD19, CD20


NJH-ADL
CD19 and CD20 percent and absolutes, Test Codes: CD19M, CD20M or CD1920
Memory B cells: CD20/IgD/CD27. Test Code MEMB
Extended B cell panel: (switching to CD19) CD19/IgM/CD38/CD21. Test Code XBCP


NSUH
B-lymphocyte subset uses CD19, CD20, CD21, CD27, CD38, CD138, IgM, and IgD. Percentages are reported. Test Code: CVID


UCLA-UIC
The B-cell panel includes a basic panel for lymphocyte enumeration, which reports the percentages and absolute numbers of T cells (CD3+), T-helper (CD3+, CD4+), T-cytotoxic (CD3+, CD8+), CD4:CD8 ratio, natural killer (CD16+CD56+, CD3-), and B lymphocytes (CD19+), followed by an advanced panel to identify Memory (CD27+), IgM Memory (CD27+IgM+IgD-), Unswitched Memory (CD27+IgM+IgD+), Switched Memory (CD19+CD27+IgD-IgM-) and Immature (CD21low) CD19+ B cells. Test Code: B-CELL