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The science and practice of human immunology

The science and practice of human immunology
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NEUTROPHIL FUNCTIONAL TESTS

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Bactericidal Assays and Chemotaxis DHR/Oxidative Burst

 

Bactericidal Assays and Chemotaxis

NJH-ADL
Bactericidal assay.  Analysis of the ability of isolated neutrophils to clear S. aureus – manual method. Test Code: BACT

Neutrophil chemotaxis.  Modified Boyden chamber method utilizing peripheral blood and zymosan activated serum as chemotactic agent. Readout is distance migrated in microns. Test Code: CTX

DHR/Oxidative Burst


ARUP
Neutrophil Oxidative Burst Assay (DHR). White blood cells are incubated with dihydrorhodamine 123 (DHR) and catalase, then stimulated with Phorbol 12-Myristate 13-Acetate (PMA). Dihydrorhodamine oxidation to rhodamine by the respiratory burst of the cell is measured by Semi-Quantitative Flow Cytometry. Test Code: 0096657


BCH-CFCL
Neutrophil’s Oxidative Burst, which reports: Neutrophil Oxidative Index (MFI DHR+ / MFI DHR-), and % Responding Polymorphonuclear Cells. Test Code: DHR assay


CALGARY-CLS
Functional CGD screening assay, using DHR123 loaded neutrophils pre and post stimulation with PMA to induce oxidative burst reaction. Test Code: NFUN


CCHMC-DIL
Classic dihydrorhodamine (DHR) 123 assay.  Test Code: Neutrophil Oxidative Burst (DHR)


CHLA-DIFL
Chronic granulomatous disease diagnostic screening assay for oxidative burst using dihydrorhodamine (DHR) 123. Test Code: CGD Flow


IOWA
Classic dihydrorhodamine (DHR) 123 assay with PMA stimulation. Test Code: SPL8


MAYO-ML
Classic dihydrorhodamine (DHR) 123 assay. Test Codes: DHR (for both PMA and fMLP stimulants); #62764; DHRP (only for PMA); #62765; DHRF (only for fMLP); #62766.


MCW-CIRL
Classic dihydrorhodamine (DHR) 123 assay with PMA and fMLP stimulation. Test Code: NEUOXB


MONTREAL-SJH
Neutrophil’s oxidation (DHR) by flow cytometry. Test Code: Activité Oxidative des Neutrophiles


NJH-ADL
Classic dihydrorhodamine (DHR) 123 assay. Offers NBT assay as back up. Test Codes: DHR; NBT


NSUH
Neutrophil oxidation assay uses dihydrorhodamine (DHR) 123 and PMA. Report includes MFI ratio. Test Code: DHR


QUEST
Neutrophil Function, Oxidative Burst.  Classic dihydrorhodamine (DHR) 123 assay. Test Code 37523


UCLA-UIC
Classic dihydrorhodamine (DHR) 123 assay with PMA stimulation. Test Code: GOB


VIRACOR
Oxidative Burst:  Whole blood is stimulated with PMA or buffer alone, Formation of the reactive oxidants during the oxidative burst is monitored by the addition and enzymatic oxidation of a fluorogenic substrate, DHR 123. The level of reactive oxygen radicals is determined by flow cytometry. Test Code: 403002