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The science and practice of human immunology

The science and practice of human immunology
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T CELL FUNCTION TESTS, INCLUDING RESPONSES TO MITOGENS/ANTIGENS, AND ASSESMENT OF CYTOTOXICITY

 

ARUP
Lymphocyte Mitogen Proliferation. 3H Thymidine incorporation assay measuring lymphocyte proliferation to the nonspecific mitogens phytohemagglutinin (PHA), concanavalin A (Con A), and pokeweed. Test Code: 0096043

Lymphocyte Antigen and Mitogen Proliferation Panel. 3H Thymidine incorporation assay measuring lymphocyte proliferation to phytohemagglutinin, concanavalin A, pokeweed mitogen, Candida antigen, and tetanus antigen. Test Code: 0096056

Lymphocyte Antigen and Mitogen Proliferation Panel with Cytokine Response. Cell culture and multiplex bead assay, which measures lymphocyte proliferation and cytokine release following stimulation with Candida and tetanus antigens and the mitogens phytohemagglutinin, concanavalin A, and pokeweed mitogen. Test Code: 20131174


BCH-CFCL
T cell Mitogen-induced Lymphocyte Proliferation (Phytohemagglutinin, Concanavalin A, Anti-CD3), and T cell Antigen-induced Lymphocyte Proliferation (Candida Albicans, Tetanus Toxoid). Test Codes: T cell Mitogen Proliferation and T cell Antigen Proliferation


CALGARY-CLS
Ability of mononuclear cells to proliferate in response to PWM, ConA, PHA and StaphA is assessed by flow cytometry cell counts and reported as a proliferation index. Test Code: MSA


CHLA-DIFL
3H Thymidine incorporation assay measuring lymphocyte proliferation to Phytohemagglutinin, Concanavalin A, pokeweed mitogens, and several antigens including CMV, HSV, VZV, ADV, Candida, CD3CD28 and tetanus antigens. Note: CD3CD28 induced proliferation by special request. Test Codes: PWM Blastogenesis, ConA, PHA Blastogenesis, CMV Blastogenesis, HSV Blastogenesis, VZV Blastogenesis, Candida blastogenesis, ADV Blastogenesis, Tetanus Blastogenesis.


CINCINNATI-DIL
3H Thymidine incorporation assay measuring lymphocyte proliferation to the mitogens Phytohemagglutinin (PHA), Concanvalin A (Con A), and Pokeweed (PWM).  Test Code: Mitogen Stimulation

3H Thymidine incorporation assay measuring lymphocyte proliferation to the antigens Candida albicans and Tetanus/Diphtheria toxoid. Test Code: Antigen Stimulation

CD8+ T cell degranulation (CD107a) following exposure to anti-CD3 coated P815 cells. Test Code: T cell degran


IOWA
Lymphocyte Proliferation to Mitogens. 3H Thymidine incorporation assay measuring lymphocyte proliferation to the mitogens Phytohemagglutinin (PHA), Concanvalin A (CON A), Pokeweed mitogen (PWM), Interleukin 2 (IL2) and anti-CD3. Test Code: SPL3

Lymphocyte Proliferation to Antigens. 3H Thymidine incorporation assay measuring lymphocyte proliferation to the antigens Tetanus/Diphtheria, Candida albicans and alloantigen. Test Code: SPL4


MAYO-ML
Flow based methodology measuring lymphocyte proliferation to the mitogens Phytohemagglutinin (PHA) and Pokeweed (PWM). Test Code: LPMG; #60591

Flow based methodology measuring lymphocyte proliferation to the antigens Candida albicans and tetanus toxoid. Test Code: LPAG; #60592

Flow based methodology measuring lymphocyte proliferation to an anti-CD3 panel (soluble anti-CD3, aCD3+aCD28, aCD3+ IL-2). Test Code: LPA3P; #62205

CD8 T cell degranulation (CD107a) and measurement of IFN-g after CD8 T cell activation with PMA/ionomycin. Test Code: GLIC; #89317

CMV-specific CD8 T cell immune competence is evaluated using MHC class I tetramers (flow assay also includes a quantitative component for CMV-specific CD8 T cells for 5 HLA alleles – A1, A2, B7, B8 and B35).  Functional component includes CD107a/b expression and IFN-g production of CMV-specific CD8 T cells. Test Code: CMVC8; #88826


MCW-CIRL
Flow-based methodology.  CFSE-labeled T cells are assessed for their ability to proliferate to mitogens: ConA/IL-2, PHA/IL-2, PMA/Ionomycin and anti-CD3 with and without IL-2. Test Code: TMITO

CFSE-labeled T cells are assessed for their ability to proliferate to cytokines: anti-CD3, anti-CD3/IL-2, anti-CD3/IL-7, anti-CD3/IL-15, anti-CD3/anti-CD28. Test Code: TINTL

Cytokine Production by Mononuclear Cells in Response to Antigen and Mitogen Stimulation. Antigens (Candida albicans, tetanus toxoid) and mitogens (phytohemagglutinin, concanavalin A, pokeweed) are tested independently. Peripheral Blood Mononuclear Cell (PBMC) cytokine production responses to these antigens and mitogens are determined by quantitative multiplex bead assay. The following cytokines (and receptor) are reported: IL-2, sIL-2R (sCD25), IL-4, IL-5, IL-10, IL-13, IL-1b, IL-6, IL-17, TNF-a, and IFN-g. Test Code: 2013111


MONTREAL-SJH
T cell proliferation after PHA and anti-CD3 (OKT3) antibody stimulation by flow cytometry. Test Code: PHA, OKT3

Degranulation CD3+CD8+ blasts (cytotoxic CD8 T lymphocytes) by flow cytometry. Test Code: Dégranulation des lymphocytes T


NJH-ADL
3H Thymidine incorporation assay to measure lymphocyte proliferation to Phytohemagglutinin (PHA), Concanvalin A (Con A), and Pokeweed (PWM) (3 concentrations each).  Available separately with Test Codes: LPHA, LCONA, LPMW, or together: Test Code: LSTIM  

3H Thymidine incorporation in response to tetanus toxoid (3 concentrations) and Candida. Test Codes: LCAND, LTET


NSUH
Lymphocyte proliferation to Mitogen uses 3H thymidine incorporation method to measure lymphocyte proliferation to PHA, ConA, and PWM. Test Code: Mitogen

Lymphocyte proliferation to Antigen uses 3H thymidine incorporation method to measure lymphocyte proliferation to Tetanus toxoid and Candida albicans. Test Code: Antigen


QUEST
Mitogen- and Antigen-induced Lymphocyte Proliferation Panel.  Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Phytohemagglutinin (PHA), Concanavalin A (Con A), Pokeweed Mitogen (PWM), Candida, Tetanus Toxoid, Tuberculin PPD (TB PPD).  Test available by prior arrangement only. Test Code: 91978

Mitogen-induced Lymphocyte Proliferation Panel.   Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Phytohemagglutinin (PHA), Concanavalin A (Con A), Pokeweed Mitogen (PWM).  Test available by prior arrangement only. Test Code: 91976

Phytohemagglutinin (PHA)-induced Lymphocyte Proliferation.  Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Phytohemagglutinin (PHA).  Test available by prior arrangement only. Test Code: 91980

Concanavalin A (Con A)-induced Lymphocyte Proliferation.  Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Concanavalin A (Con A).  Test available by prior arrangement only. Test Code: 91982

Pokeweed Mitogen (PWM)-induced Lymphocyte Proliferation. Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Pokeweed Mitogen (PWM).  Test available by prior arrangement only. Test Code: 91981

Antigen-induced Lymphocyte Proliferation Panel (Candida, Tetanus, TB PPD). Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Candida, Tetanus Toxoid, Tuberculin PPD (TB PPD).  Test available by prior arrangement only. Test Code: 91977

Candida-induced Lymphocyte Proliferation. Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Candida. Test Code: 17270

Tetanus-induced Lymphocyte Proliferation. Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Tetanus Toxoid. Test Code: 17274

Tuberculin PPD-induced Lymphocyte Proliferation. Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Tuberculin PPD (TB PPD). Test Code: 17680


VIRACOR
T cell Function Analysis: PBMC isolation from whole blood, stimulation with various antigens or mitogens, flow cytometry analysis for CD69 upregulation and interferon gamma production. Test Codes: 403066 and 403065

Intracellular Cytokine Panel:  Whole blood is stimulated with PMA and ionomycin. Following stimulation cells are stained with antibodies to identify the cell population (CD4, CD8, NK and NKT cells) and cytokine (interferon gamma, IL4, or TNF) of interest. Test Code: 404474